Studies
|
| Veterinary student restraining calf | Veterinary student |
Cows from one of the farms on which the calf study is taking place |
| Veterinary student collecting fecal sample from calf | Dr. Michael Bolton processing fecal samples in the laboratory |
TREK machines used to perform liquid culture of feces |
Michigan Johne's Disease
Herd Prevalence Study
This
study is being conducted by the Michigan State University
College of Veterinary Medicine. The objective is to determine
the prevalence
of Johne’s disease at the herd level in Michigan Grade
A dairy farms using a novel herd screening strategy. This approach
being used involves sampling and culturing of bulk milk and feces
taken from the primary manure storage areas.
One
hundred and twenty (120) dairy farms have
been randomly selected
to participate in the study. Farms were proportionately
selected
from different regions of the state
based on number of farms in that region. Milk samples are collected
from the bulk
tank
and fecal samples are collected from two locations; the primary
manure storage area and a common cow area. These samples will
then be cultured for the presence of the bacterium that causes
Johne’s disease, Mycobacterium avium subsp. paratuberculosis.
The data collected from these tests will be evaluated and compared
to determine the prevalence of Johne’s disease in Michigan
dairy herds.
Herd Prevalence Study Images
Veterinary
Student collecting a manure sample |
Veterinary
student collecting milk sample from bulk tank |
Veterinary
Student collecting manure sample from a lagoon |
Economic Study - Under Construction
This study investigated the survival of Mycobacterium avium subspecies paratuberculosis (the causative agent of Johne's disease) in recycled sand. Please read the abstract for further information.
Abstract: Mycobacterium avium subsp. paratuberculosis and its Survival in Recycled Sand
Mycobacterium avium subspecies paratuberculosis (the causative agent of Johne's disease) has been incriminated as the possible cause of Crohn's Disease in humans, though this is still under investigation. Should this be confirmed, outside the economic loss associated with Johne's disease, it is of public health importance. Culturing of MAP has remained the most sensitive confirmative diagnosis of Johne's Disease, however it takes at least 6 weeks to get the result, thus the need for a Biosensor; which would provide the result in less than an hour.
A biosensor is a device that incorporates a biological recognition (sensing) element in close proximity or integrated with the signal transducer, to give a reagentless sensing system specific to a target compound (analyte). Typical biological recognition elements used in biosensors range from DNA probes, antibodies, viruses, antigens, enzymes, cells and tissues. The methods of signal transduction used range from electrical, optical, electrochemical, thermometric, piezoelectric and magnetic. There are a wide variety of applications for biosensors, such as homeland security biological countermeasures, clinical diagnosis, food and water safety, fermentation control, bacterial and viral analysis, industrial effluent control, military operations, and space applications.
Our research focus is to develop conjugate strip biosensors for rapid and on-site detection of MAP in cattle for the ultimate purpose of early detection of disease and control thus enhancing possible public health.
Likelihood
Ratio - Under
Construction
Abstracts and Papers
Mycobacterium avium subsp. paratuberculosis and its Survival in Recycled Sand
Survey of Management Practices to Control Johne's Disease on Michigan Dairy Farms
Publications
1: Michael W. Bolton , DVM, Daniel L. Grooms , DVM, Ph.D,
John B. Kaneene1, DVM, MPH, PhD.
Fecal shedding of Mycobacterium avium subsp. paratuberculosis in calves:
Implications for disease control and management.
Proceedings of the 8th International Colloquium on Paratuberculosis(Pgs 596-600)
Copenhagen, Denmark in August 14-18, 2005. Published in Full - 8April, 2006
2: Dunn JR, Kaneene JB, Grooms DL, Bolin SR, Bolin
CA, Bruning-Fann CS.
Effects of positive results for Mycobacterium avium subsp paratuberculosis
as
determined by microbial culture of feces or antibody ELISA on results of caudal
fold tuberculin test and interferon-gamma assay for tuberculosis in cattle.
J Am Vet Med Assoc. 2005 Feb 1;226(3):429-35.
PMID: 15702695 [PubMed - indexed for MEDLINE]
3: Johnson YJ, Kaneene JB, Gardiner JC, Lloyd JW, Sprecher DJ,
Coe PH.
The effect of subclinical Mycobacterium paratuberculosis infection on milk
production in Michigan dairy cows.
J Dairy Sci. 2001 Oct;84(10):2188-94.
PMID: 11699450 [PubMed - indexed for MEDLINE]
4: Johnson-Ifearulundu YJ, Kaneene JB, Sprecher DJ, Gardiner
JC, Lloyd JW.
The effect of subclinical Mycobacterium paratuberculosis infection on days
open
in Michigan, USA, dairy cows.
Prev Vet Med. 2000 Aug 10;46(3):171-81.
PMID: 10913802 [PubMed - indexed for MEDLINE]
5: Johnson-Ifearulundu Y, Kaneene JB.
Distribution and environmental risk factors for paratuberculosis in dairy
cattle herds in Michigan.
Am J Vet Res. 1999 May;60(5):589-96.
PMID: 10328429 [PubMed - indexed for MEDLINE]
6: Johnson-Ifearulundu Y, Kaneene JB, Lloyd JW.
Herd-level economic analysis of the impact of paratuberculosis on dairy herds.
J Am Vet Med Assoc. 1999 Mar 15;214(6):822-5.
PMID: 10101415 [PubMed - indexed for MEDLINE]
7: Johnson-Ifearulundu YJ, Kaneene JB.
Management-related risk factors for M. paratuberculosis infection in Michigan,
USA, dairy herds.
Prev Vet Med. 1998 Dec 1;37(1-4):41-54.
PMID: 9879579 [PubMed - indexed for MEDLINE]
8: Johnson-Ifearulundu YJ, Kaneene JB.
Relationship between soil type and Mycobacterium paratuberculosis.
J Am Vet Med Assoc. 1997 Jun 15;210(12):1735-40. Review. No abstract available.
PMID: 9187720 [PubMed - indexed for MEDLINE]